CR71562

CRIMIC CR71562

Gene IntS12

CG5491

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:26863376..	97D3-97D3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGAATCCAATACAACCAACGTGTG	CGGAAATACCAGGGATAGGAGC

Images

Construct Design Sequences

sgRNA sequence	TGTTATTTACTATATCTACCCGG
sgRNA2 sequence	GCCGCTTTCGTGATGCTTGGAGG

left_200bp	right_200bp
ACTAACATTGTTGTTAACAAAGCTATATTCAGATTACATGAAATGATTCATAACTTTATTTTCATCGTATTATGGCTAATAACTTAAAATTTAATTTTCCCGCCTAAAACCTATGCACGTTGCCAAGTGAATGGCTGAAACCAAACAAACAGCTGTTGCGCGGGCATAAACTTGCATTTATATTGTTATTTACTATATCT	AGCATCACGAAAGCGGCAGCAGCAGCAAGCGCAGATCCAAGCAGTAATTTAGCATCTCTAATAATTAAGTATAAAGTTACAATCCGCATAAAATTCTGAACGTTCATTCATAATAATAATTCAATAATTCATTTTCAATAAATGATTTTTAAAGATATGTACGTGCTATCGAATTCAGTAAATGTAGGTAAAAGTAAGAA