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CRIMIC CR71563

Gene asun

CG6814

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:16264271..16266891	89B9-89B9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGACAGCTTTCGCTTAACAACG	CCGTGCCAAAGATCACAATCAC

Images

Construct Design Sequences

sgRNA sequence	CGTGGACAACTTAAATTGCCGGG
sgRNA2 sequence	GAATCCGTACGTAGTTAAAATGG

 

left_200bp	right_200bp
TAAATACATCGATATATTTTTTTTAAATGTGTGCTTTTTTGTTGAATTTGAGAAAAGAGCTTTTTAACACAGTTTAGTAAATTTAAAAAAATAAAAAAAAATTAGCTGTGGTGAATTTAGAATTCGAAAACAGCTGTTCGTGGTGAACAAAGATTGTAAACAATTAGGATTCAACGTTTGCGTTTGTTTGATTTCCCGGC	AAATGGCACGGAGAACGCCGAATTTAAGCTGGGACTGTAGTATATATATATATAAAAGAAACATAATCATTTCCTTAAACAAAGCTTAGTGTACGGCCTAATAGGAATTAATTTGAATGTAAAATGAAGTATTTTTCTGAGTTTCATATAATCGCTTCTTTAAGACTGGGACAACATTACTGTGGAAGACGACATTATAA

 

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