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CRIMIC CR71564

Gene IntS14

CG4785

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:1164443..1166425	21F1-21F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCGAATTCTGCTGGAGGCGATTC	CGAATCATCGTCATCCTCGCTC

Images

Construct Design Sequences

sgRNA sequence	GCTGCGAATGGCACTTCAGCTGG
sgRNA2 sequence	AATTTAAATCTTAAAAGTTCGGG

 

left_200bp	right_200bp
ATTAGGCATATTTCGTTTACTTGTGGAGAAGGCAGTTATGTAAGCTCACTCAATAAGTAATAACCATTAGGATTGTATTTTATCAAGATTGTAAATTCAGCACTTTATGTATTTGAATTTCTATTTGGCGCCAAGCTATGGACAAAAATCATTCACTTGGAACTAGAAACCGTTATTCGCTGCGCTGCGAATGGCACTTC	TTCGGGCTTTAATGAAAGCGTAACGGTATTTTTCATCTCTGCACTTCACAGCACTGGCCGCTTGGCGTTATCGTTATCGATGACCAAATAATATAGTAACAATAAAAGTCGTAAAAATAATAATGGGAAGTCCAGAATTGCCCTCAGATCCCGGTCAAGAGGGACTTCCAACCCCACCGGTTGACCATCCACGGGGAGGG

 

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