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CRIMIC CR71565

Gene CG5274

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:20426199..20427666	77C2-77C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGTTTTTGTTTCCTTCAAAGGGAAG	CTGTGTGTCCTGTTCAGTGTTCAC

Images

Construct Design Sequences

sgRNA sequence	TGTACAAGATAAAGGACTAGAGG
sgRNA2 sequence	CAAACACTATAAACGTCAGTCGG

 

left_200bp	right_200bp
TGTACTGTTATGTGTTTTTGTTCGGATAAAAACGGATAATCAGTTTTAGAGTGACCATTGTTCTGGTTTTAATCGGTGGACTATCGAGCTGCCAATCGGAATATCTGCCACCTCTAAAGTTCCACGGTTTGTTTTTATTTTTCTGTTGCATTACCATACATTCTTAAAAACCTGTCACTGCTTTGTACAAGATAAAGGAC	AGTCGGTTAATGCGAGTAATTAATCATTAAAATTAAAATGTAGCTTTTACAAATAGATCTAAGACGTTATTAATTAAACATTGAGTCGCTTACAAATCTTTTAAGCCCAAAAATGTTTGTTTGGTTAAAAGAAGAAAGGGTTTAACTATATTAACAAACAACAATTTATACCGAATATTACATGTTTAAAACACTTTTAT

 

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