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CRIMIC CR71566

Gene aln

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:993983..995703	61E1-61E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAAAACGGAAACTCTAAACACTTGAATG	TGGTGAATGTTAGGTAACCAAGAAGAAG

Images

Construct Design Sequences

sgRNA sequence	ATATACCCGTACCTTCGATGGGG
sgRNA2 sequence	GTACGGAAGTCGGAGAAGCTCGG

 

left_200bp	right_200bp
AGGGTAAAAATGTGGCTGAAAGGAAATCATTTTCATGACAATTTCGGGTGGAGAACACTTTTAAGAAATAAAGGATAGATTGGCAGCACTGATTGCGATATACATCGATAAACAGGATTCGAGTGCGCACTGCGCATGTCAAGGATGTGTCAGATTTGGTTGACAGTTCTGGACTTTAAGGGTATATACCCGTACCTTCG	GCTCGGAAGCAGCGGACGGTAGATCAATTGGCGTACAGCTGGCTCAAAACTGATAGCATTTTTATAAGTAGACTTAGTTAATTACAAGTAAATAAAACAAAAATGCTTTTTTTGACTACAACAAAACAAATCTTATTGCCTCTTTAAATTAATTACCAATTTAATTTAAATTGGATAAATCAAGAAGCCACTGCAAAAAC

 

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