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CRIMIC CR71573

Gene fzy

CG4274

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:16310956..16313097	35F1-35F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCAGGCAGCACAGATGTACG	GGAACCTTATGTACTTACTGTCCC

Images

Construct Design Sequences

sgRNA sequence	ACAGAAGCTCATAGAACACTTGG
sgRNA2 sequence	CAAGAAATAATTGTCTAGTCTGG

 

left_200bp	right_200bp
AAATCAAATATAATTTCATTTAAAGAAGCAATTTTAATTTCTAACGAATTATAATGTTTTGTATTAATTTTAATTCTCTGGTAATTTTCTCTACGCACAAAAAATACCACTAACAGTTAGCCGATGCGGTCACACTACATTTCCAAATCGAACCGCCATTTGTGTTGCATCTCGAAATCTGGAAAAAATTCGATCCAAGT	TAGACAATTATTTCTTGAATGCATATCCAATTTTTATGTTCTCGTTTAATGTTTCGTACTTTGTAATTACGGATAAATCTCTGATTATGTGCCCCCGCCGACGATATATGAATAATTAAATTTTGTCAGTTACTTTTATTGCTTAGAGTTAAGTTTGTGTGCGTATAAATAAAATGCTTGACATTCTGCATCCTTGTATG

 

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