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CRIMIC CR71575

Gene CG13334

CG13334

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:13558545..13560768	50B1-50B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCAGTGAGACCTTCAGTCGTAT	GGAAAGGGCAAAGGGCTTG

Images

Construct Design Sequences

sgRNA sequence	TGAAACCGAAAAAGGAATACTGG
sgRNA2 sequence	TGAGCTACTGAAGAATTCTATGG

 

left_200bp	right_200bp
TATAATATAACGTTTTAATTATAACCTAAATAACTCATATAAAAAATAAGAAATCAAAAAAGTTGGTAAGCATTTTGCACAAGTTAATCTAAACAAATATGTAAGGATAAAAACTAATCCAATCTAATTAAAACAAAGAGATAGCAAGTACGTATCTTAAAAATGCGATACTAAAAATATCCGTAGGCTTAGTTCGAGTATTCCTTTTTCGGTTTCAAAATTCAAAATTAAAAA	CTATGGAAGAACTTATACTAATGGTTTATTTGTTGTTCCGAAATATTTATATCGCAAACGAATACCTTTTGGAAATGTACATATTCTGGAAAATGTATATGGTATGTACATATACAGATAAGAAAAAAGAACTTATGCCTCCATTCAATGCATTGTTCATGGAAATAGCCCATAAAAAACACACCCAAATCTCAAGTGGA

 

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