CR71576

CRIMIC CR71576

Gene tilB

CG14620

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:22449737..22451463	20C1-20C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTTAACTATGCCCATTGCAAGG	CTGTTTTGCATTATCCGACTTACGTTAC

Images

Construct Design Sequences

sgRNA sequence	AAATTGCCTAGCAACGGAGTTGG
sgRNA2 sequence	AATTAAATCGGGAAGATCTTCGG

left_200bp	right_200bp
TGTCACGCCCGCTAGTGAAAAATCATTTTTATTTGTTTTCGATATCGATATCGAGAAAAATGTCAAAATTTCAAAATTTCTCGCCCGCTTTTGAAGTATGTACATTGCTGAGTAACGAGTTTTGAATAGTATAGTATTTATTATTTTATTCTCCAATAATAATAACGAGGAATGTGGAATATAAAATTGCCTAGCAACGG	ATCTTCCCGATTTAATTTGAGACTGTGTTCATTATTTAAAAATGGTTTATTTAAATATTTGTCTGATTTGGTTTGTTTTCTCAAACTCCTCAGTGATCTTTTGTTTTGTTTGGTTTTTGTGATCCATCCATTCCATCGATCAATCATTAGCTTTCTAGTAAATACTTTAGCCCGATCGGATGCTCATATATATATGCGCT