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CRIMIC CR71577

Gene CG14450

CG14450

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:22726234..22727789	80A1-80A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACGGACTGCTCCAGTTTCTTC	GCCACTTTGGTCTATCAATTGGAC

Images

Construct Design Sequences

sgRNA sequence	CTATACAGCTGTTCGGTTAGTGG
sgRNA2 sequence	TTAATAGGAGCAGTTTCTAATGG

 

left_200bp	right_200bp
TGTGATTTATATTATATTTATAAATTATTTCCTTTGTAGTTTTACCTAAAATGTATAATGATTGCTGTGTGAAATACATGCCTTTTCTCTCATCTTTTTCACTTAACAATTAGTTGCTATAAATTGTTTGTAGCCGTCGATGGTGTAGCATTGTATATCGATTAATTAGAAAAACTGAAAAAATGAGCTTGGTCCCACTA	TAATGGCACAACTGCATTTCCATGGAATACCATAAACATAGATTATTTTGAGCTTGGCCCCACTTAGCTAATTAGAATTTGTCGCCATCAAAAATATCATGTCTTTCTTTCATTACTGCCCACATATTAGGTCTTAAAAATATTTATTTAGCAGAACTAAAATAAATAAATACTTGATGCTGGCCCTGTCCGTTTGAACG

 

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