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CRIMIC CR71586

Gene dib

CG12028

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:4062292..4064551	64A5-64A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCAAATCAAAGCTATACTTGGGG	CCGTTGTTCGAGTATTTGGCAACC

Images

Construct Design Sequences

sgRNA sequence	GGCAAATTCTCGAAATGCACTGG
sgRNA2 sequence	CTGCGATTGCGTAGAGAATAAGG

 

left_200bp	right_200bp
AAGTATACCTTTCTTTTTAAGAATATACTATTCCCATTTTGGCTCTCTTAATTTAAAGAGCGAAAACATCGGAAGTAAACCCTCTCAGGTAGGTAGATTCACCACAGGTAGGCACAACTTCGATCCCAGTGTGCGTTTAATGCCTTTTTCCCGAATGCTGAGCCGGCAATAAAACCCTCAGACCCATGTAGTCTCCAGTG	ATAAGGTTATTAGGTATAAGTAAGTCGCCAGAGCCTTAAGACTGAGATACTAGACTCGTGTCACGTTTAAATAATTCGTTTATTAATTATTTTAAATTAGCTCATAATTAATTATAATTAGTGTAAATTATATATAACTAGACTTGCATTTTATGTGATTGAAAGGCTGTGGGTGTTTTGGTCTAAGTACTAAAGAGAAC

 

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