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CRIMIC CR71592

Gene SERCA

CG3725

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:23925077..23935884	60A11-60A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	OK	CACCTGTGTATGTGCGTATGTTGG	CCACCAGTCAATTCTGTGACACC

Images

Construct Design Sequences

sgRNA sequence	TCGTTATCCTCTTCGTTCAGTGG
sgRNA2 sequence	AGTCCTATATATAAGATGCATGG

 

left_200bp	right_200bp
TGCAAACGTACATGCCAGTACAGATGTATGTGCATACATACGTGCATACACACTTGCTATTCCAGTCAGTCGTTGAATAGAGCCCAGAAAACGGCAAAATAGCTAAATAGCCCTGCCAGAGAATTAAAATGAGCCCCTTGCCTTGTGTCTACTTTCAAATCCCCACCTGAATCGCCCCGGAATCTGGACACAGGCCACTG	GCATGGGATTGTGTTAATGTGGGCTGTCTTCTTTGGCCTGCTGTACGCTATGATGCTTTAAGAATATAATTATAATCCATAAGCCCAACTTCATTATTCTAATTTCTAGTATAAAATGTTACTAAATATAAACAGAAACAAGAACCCACAATTATATACATCTAACGTAACGACTTTAACCCCATGAAGTTTGTTCCCAA

 

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