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CRIMIC CR71595

Gene BCAS2

CG4980

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:37921283..37922356	98B6-98B6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCAGCAAGTGTCAAGCTTACAG	CACCTTTGTAGTGGGCCATCCTT

Images

Construct Design Sequences

sgRNA sequence	TCTGCCACACTAGTGCGAAGCGG
sgRNA2 sequence	ACGACTGTAACTAAATATAAAGG

 

left_200bp	right_200bp
ATCAAACAATACATAATACATACATACGTTAAAAACTAACTTACACAACTTTTATTCGTGAGAAAACATCGCTTATTTTTTGCTTAAATGTTCATGTTAAAACAAAAAAATTTACTAAACAGATATATGGCTATTAATTAACAGCATTCTGTTATCCGTTTATATAGCTATAAAGTAGCTAAATCTGCCACACTAGTGCG	TAAAGGATATTAAAAAAAACAGCTAAAAATCTAAGATTTATCTTTATTTTTCAAAATCGTTGCAGAGAAATAGTGATCCTCTTCTTTTTGCTCTTATATCAGGGGAATGGACAGCTAGTAAATTTTAAATAATTTCAAGATTAAATATTTTATTATTTCAAATTGGTAAGGTAGTGTTTTGTGCTGTAAATTTTTTATAC

 

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