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CRIMIC CR71599

Gene alphaCOP

CG7961

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:1641066..1645199	62A9-62A9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTATGCACATCTGCCAACG	TGGACAACTGAGAATGCTCCGAA

Images

Construct Design Sequences

sgRNA sequence	TTGGATGCACTTCTAGGTGTCGG
sgRNA2 sequence	AACTTTATACATAATAAGCAAGG

 

left_200bp	right_200bp
CGCGAAGAAGAAGATAATTGACGTGTCACAATTTCGACGATTGGTTGGTGTTTTTCgTAAAATTAATTGTTGGCAGTATATTACGTGTTACTTGTAGCGTATCAATAAAATGGGTTTGTTCTAACACCCACCTCTTGATGTTTTGATACAGGCAAGTGCTCGAACCAGCAAAAAAAGGGCGCTTGAaGGATAAGCCGACA	TTATTATGTATAAAGTTCAGTATCTGTATATTATAATGCTATAAAATCAAAtCAAACGACTTGTTTTCTTGcTCGATACCTAGCCTCGGTAGGCAATTCCCAGTGAAGAGTCTTAGTCACTCTGCTTAAAtaTGGAAATTCCAAAATGAACAATGCCATATAGATTAGTAATTATGTATTTTAGTTAAAGATACGACACT

 

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