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CRIMIC CR71610

Gene Dso2

CG33990

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:20870392..20870678	57B3-57B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGTGCTTAACAATGATGCTCTG	CAGCTTGCTTTTTCCACAAGTCGC

Images

Construct Design Sequences

sgRNA sequence	TCTACTTTGATTAGCGATCCGGG
sgRNA2 sequence	ATTACTTAAACATTTATTTGAGG

 

left_200bp	right_200bp
AAAACCTTTATCGCGTGGCAGTGCATTTAGTAGTATTGTCTCGTATCAGCTAAACGCGCATTATCAGCTCAGATCGCGCTCAATTTCCTATCGTGACCCTGGGGAATTCGAAACCCCCTGGAAATTCCTACTTTTAAAGCCGCCAGCTGGCGAGCTCACTGCATCGGATGGATGAGCTATATAAGCGCGCCCTGCCCGGATCGCT	ATAAATGTTTAAGTAATAAAAATGTTTTGAAACAGTAAAATTTCCTATGTTTATAAATTTGAAATGAGCGCGCACATAGTGGGAAAGGGTCACACTGGCTAAATATACTAGATTGCTGAGCATAAGCAAATTCTATGTTCTGGTGTGCACACACTGCAAATGATCTCAGCTGATCTGCTGTTCGGAAAACGAAAATAAAC

 

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