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CRIMIC CR71614

Gene eIF3f1

CG9769

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:4712882..4714182	82D2-82D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACAACACGCAGGCGACTTGATGT	GTGCCGAAGGTACGATCATCAAAG

Images

Construct Design Sequences

sgRNA sequence	TTCGGTGGCCAAGATTTGCAAGG
sgRNA2 sequence	ATGCTCCTACAAACGCCACATGG

 

left_200bp	right_200bp
TATTCAAATTTGAATAGTATTTGATTAACTAACGCTAGCCACCGGTTTTACATCGATAAAACGATAGGCCACGCACGACCGCACCGATAGCTCCAAAAGCCATCAACCCCCACCCGCACTATCGATAGGCCCTTTTCTTGCATCCCTAAAACATACGCGCAGCGTGGCTGAATAGAAATTATTGCGGAAATTTTCCTTGC	GGCGTTTGTAGGAGCATTCGAATCTACTGAAGACTCTTAAACACAACAGTATTCATCACCAGGAACAAAATAAATGCATGTGCAACTGAAAATTTAGCACCCAACTGCTAAAACAAAATGTTGTGTTTCAGTCTTTGGTGAGCTTCACTATTCTATAGGGTTTCAGGGAGTCGCATCGGTGCTGGGTTTATTGTAAGAAT

 

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