CR71617

CRIMIC CR71617

Gene Ski6

CG15481

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:13221397..13222429	34A10-34A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGATATCGATTAGACCATGTGTTAGTG	CTGGAACATCGAAGGGAACGACTA

Images

Construct Design Sequences

sgRNA sequence	CTAAAGTTTCAAAATATGTCTGG
sgRNA2 sequence	ATTGTAAAAAACGACAAGATTGG

left_200bp	right_200bp
CAATATATGCTTAATTATTTATTTACTCTTACATACGTACGGTGTACATATTAAAAGAAGTTAGTATAAAAAGGATTTTTTCATGGACTGTCGGTATTTTTAGTACTTCCACACTGTTGTTAAAATTGTAAACAAATCCCGCCGCACGTGTGATATTTAGTTAAAATCAAAATAATTGTATTTCTAAAGTTTCAAAATAT	TTGTCGTTTTTTACAATTGAAGGGCAACAATTATAATAAAAAGGAACCAACATTTACAAATTAAATAGTGTCTTTAACATTGTATTCAGATGGCTGCCCTTGCTCTCTTTTAATATAATTTCTGTTGCAAAAGCACAATTATCCTCTAAAACACAGGCGTATAAATGTAGTAACAAACGAAAGTAGCTCTAGTTTTTCTC