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CRIMIC CR71618

Gene beta-PheRS

CG5706

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:24250460..24252679	95F5-95F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATTCCGATCCCATTTGCCCAATC	CACACATACTATATGAGTATACTACGG

Images

Construct Design Sequences

sgRNA sequence	TAAATTGGCAACACTGGCACCGG
sgRNA2 sequence	GCTGACCACGCCTTGTTCGGCGG

 

left_200bp	right_200bp
GAGAACTTAAACGTAACTCAAAATTACCCCTGAGCTTAGTGTGAACGAGTCGATTTATTTAGTTTTCATCAATCTCTTAGTGTGCACAATCTATTTTATGGAAAATAGTGTGACCCTCCTGCATAAGTCAGGGTGACCATCAAAACATCGCCCATGCGATAGTACTGCAAAATAAGGTCACCATAAATTGGCAACACTGG	CGGCGGTGGAATTCACCATTGAGCCTTTCGTCTAAAAGATTTGCAAAATGTTCAACATTGTCTAAAAGTTCACAAATAAATTATCACAAATTAATAAATAATGCCAAGGTTTTGTTATTTATGAAAGCCGAAACCCCAAGCCAGTTCAAACAAAACAATTTGTTGTTATTTTTTATTTATGCTGTTTAATGTTCTCTTTT

 

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