CR71621

CRIMIC CR71621

Gene Mtpalpha

CG4389

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:9573563..9576769	30B12-30B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTACTTGCAGTCTAAAACAAGTACGAA	ACGCTGCTATTGAAACCTAAACTATAGA

Images

Construct Design Sequences

sgRNA sequence	GGTACGAACGCGGTCACACTGGG
sgRNA2 sequence	AAATTTCGTTAGCGGCTCTACGG

left_200bp	right_200bp
GCTACAATAGTAACACTAGTTATGATCTCCTGCGCCACCTAGTTAAAAAAAAAACGACACTAGCGACACCTGGTGGATGAGTTTGTACTGTGTGCGGTATTTAGGCTAGAGCTAAAATTTAGAAAATACTTATGATGGTATATTTTATTGGTATGAGCGCGGTCACACTGGGGATGTGCAACAAGTAGAAATTGCCGCAT	AGCCGCTAACGAAATTTAGCTTTTAGTGCGAAGATAAGTCGTGTCCTTGTGCATTTCTCTCAGAAAGGAAACCTATTGTGGTCCATCGCTGCCTAAAAAATGGGGCAAGGCCCAATAAAAAGCCCTCTGTTGAAGTTGTCGACAATTAAATTAAAGTTTACCGTGTAATTTTCCATACTTTCATTTGTTTATTATTACAC