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CRIMIC CR71622

Gene CG3663

CG3663

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:24661503..24662409	60D13-60D

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTAGTCTGTGTAAGCATCTAGC	CTCATCCCACACTGCTGCACAAAT

Images

Construct Design Sequences

sgRNA sequence	TTCACACTATTTACTAGATTCGG
sgRNA2 sequence	AACAGATATTTAGACAGGAGGGG

 

left_200bp	right_200bp
AAATAAAAGTTTGAAGTTTGAATATCAGTTACAGCCATGTACTAAATCTTCCATTAGGTAATCGATAAGTGCTTTGCTTATAATTCTTATCGGTATCGATCAAGCCAACCGTTTCGTTTTTTCGTACCCCTGGTAGTAAACAGCTGTTACAATTTGCAGTTTATTTAACAAGTGAACATTGCGTATTTTCTAAGCCGAAT	CTGTCTAAATATCTGTTTGTTATAAAGCCAATTGCATTTATAAAACCATCATGCAGAATTGTGCTTTTCAAGGATACGCGGATGGGAAATGGGCCGAATGGGCCGAATGGGAGCAGCACCCATTTAAGTTGTCATTTCCGTAACATATTCCAATGGCCCCGCACAATGGGCGAGTCCATATTTGAATTTGCCGTTTAACA

 

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