CR71623

CRIMIC CR71623

Gene dbe

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:868673..869857	21E2-21E2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAAGGTGTCAGGGCTAAATCATG	TAGCGGGCACCGCAATAGAATCAA

Images

Construct Design Sequences

sgRNA sequence	TGGCCAGGCTGCCAGATCAGCGG
sgRNA2 sequence	CCTGAAGGCCAAGCTGATCAAGG

left_200bp	right_200bp
CAGCGGTAAATGCGTGTGACCATATCAGGGAAAACTGGTTGGTTAATATTATTGATAGATTACCTATTTAAAGGTTTATTTTTATCTTGATTATAAAATAAATTTTTCGATTGAGAATGTTTTAAAATATATATTCAAATACAATTATATTTGATCGTGTGCCAGATAAATGCCTTAACAAAATTGCGATTCCAATcTAA	TCAAGGCCAACAAGAAGGCGAGGAGCAGCTGATAGTTTTCCTAGTTTTAATTAGTAGTAGGACCTATTCAATAAAAATTGATTTTCTGAGCAACGTGTAAATAGAAATCTTAAGGTTTACTAATGGGATGAAAAGTTTGTGGGGACGAACTCGTATTAATTCTAtTTGTTTTTAAAAGCCAATCTCaAAAAATGCAATGG