CR71627

CRIMIC CR71627

Gene LTV1

CG7686

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:10836570..10838727	47C3-47C3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGTAGATGTAAAAGAAATTTGATGTATGTAAGAG	CGGCACAGTCAGCCACTTTGATTA

Images

Construct Design Sequences

sgRNA sequence	TTACGAAAACAATCCAATAATGG
sgRNA2 sequence	AGTTTCCGGCTCTGTTCTCTAGG

left_200bp	right_200bp
TCGCATACGTTCAATTAATAATAATCCATTGGCGCCTTTTTTCGAATTTGAGTGTGCGTTGTTCAACACTAAGGCCAACTGCGCAGTGAAAAAAACAGTGTGACCGCAAGGCAGTAATACAAAATCCCTTTCTGGGTGGCAGCTCCAGCAGGCACATGTGTTTCTCTGTATTTACAAAAGATTTTACGAAAACAATCCAA	TCTAGGCTTAAATGTGAAATGTATACACAGCGGCGTATATGTTAAGTGATATCTATAAACGAACGATTGGATAAACGACAAACTGTTGCCGTGATTGCGGGCATAGTTCACCCGAATTCAATGCACTAGCATAAGGAGTTGGAGACGGCACGGGATGTCGCCTTCCTTTGGCGTCATCGTCGGTTGCCAGTTGTCAGTCG