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CRIMIC CR71628

Gene CG32371

CG32371

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:7767269..7768352	66A12-66A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGAATACACTCACCTCATGCTGG	GTCCATCTCATAGGCCAGGTCATA

Images

Construct Design Sequences

sgRNA sequence	AATACTCTGATTTGCCGAACCGG
sgRNA2 sequence	CGATTTGAATGCAGTTTATTTGG

 

left_200bp	right_200bp
GCATGGGCTCCATCTTTTACGCCTCGGAAAGTATTACCTCCCAGCTGGGCTATCTGCCGGTAAGTTAGAAAAAATACAATACTATACGATATACAATGAAAACTTCCAAATCAGTAGGTTTTAAATTTAAGAACATAGTGAGAGAAAAATCACTCATAGTATGTTTAGAGAATTAGTTCAGTTCAAACCTTATACCGGTT	AAACTGCATTCAAATCGGCTAAGAAGCAAAATTCAAAGCAATGTAGTTAGTTAAAAATCATAAGTTAATCTGTCAATAAATTTTTAATACGAAGTTTTTTCTAATCTATTACAAATTTATGGGAGTATGTAATTATATATAAAAATGAAAGTAGTGGAATTTAATATTATATTTTTCTGATGTGCAAAGTGAAGGACCTA

 

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