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CRIMIC CR71629

Gene EB-SUN

CG18190

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:18851907..18853043	55F7-55F7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATCCTGGAAATGACTAGGTACCC	CACATTTGGTGGACTCAAGCGACA

Images

Construct Design Sequences

sgRNA sequence	TGATCCTGGAACATTACAATCGG
sgRNA2 sequence	TGATGAAGGCGCCGAACACGCGG

 

left_200bp	right_200bp
AATAATAATTTGGTTTAGTTTCTATGGAACGCATAGATTAAATTTTTAGAAGAATAATCGGGACATAGTTTAATTTTTATCATTATCGGTATTTTTTAAATGTTGTACCTCAGCAAACACAAAATACTGAAAAGGGTACCTTGTTTTAGGGCCAGGTACACGCGTGAAGTTACCTCCCATCCCTGATCCTGGAACATTAC	ACGCGGATCCCAATGACGGATTATATTAGAATTCTTTTTATTTGTACTTATTTTGTTTTATACAATCATTTAATTATTTATAAAGAAAGCATTATAAAATCAGCCGTTGGAATCATTGTCTGAGGAGGAGTAATCCCCCACTAAGCTTATGGGTTTTTCGTTCGTGGCTGAAGATGTAGTTTCTTCCAACTTTTTACGCC

 

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