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CRIMIC CR71630

Gene Mst27D

CG31907

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:7059214..7060851	27D7-27D7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTCCGAGTTCTTGGATTTCTGG	CGGAACAGGAAGCAAAGGAAGCTA

Images

Construct Design Sequences

sgRNA sequence	CAGTTATATTAACTTGGGTAAGG
sgRNA2 sequence	TGCGTCCAATTTAGAATCCACGG

 

left_200bp	right_200bp
TAATAATAATAATATGAATAACTTGTAACGAGCACGAAACCCACAATCGGAGTAGGCTGTGACCCTGTTTTCTTTCTTCGGGGAATTCCATTTAGAATGTACCTCAGTCAACACAGAATCGGTAAGAATAGCTGAACTTTAAAAGTAGACTTAACTATAAATTAAAATTGTTGTTTCCTTTTAAGACCTGTACTCCTTAC	CCACGGAATATACAAACCACGTGAATGTAAGCATCTAAAGAAGAATTACTCCATCGGATACGATTTTGATTTTCATACATAACACCTTATGAAAAAAAAATGTCTGCAAAATTTCGTTCCAAATGAATTTCAAGAATTATATATTTTCTATTTTCAACTGGACTGAAGTGTTAAATAAAATTTATAATGTGAAGTAATGA

 

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