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CRIMIC CR71631

Gene CG15306

CG15306

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:10330160..10338181	9B7-9B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAAAGCAACAGAACTACACATTTCC	CACCTAGCGGACATTAAGGGAAC

Images

Construct Design Sequences

sgRNA sequence	CATAGAGAATTCGCTTGCGATGG
sgRNA2 sequence	CGACGACGTCACGATATCAGTGG

 

left_200bp	right_200bp
TTGGCTTACGCGCGCTGCATGATATTTTACATTTTTCCAAAATTTTCCAAAAGCAACTTTCTTGCGAAAAAACTAATCGAACAAGGCAAACCATACGTGCTCCTGTACAACTTTCTTTGGGTAAGTGATTGTTGAATGACCAAGTAAGACATCGCTAATGTATTTTTAAGTAGCTTGTAGACACATAGAGAATTCGCTTG	ATATCGTGACGTCGTCGGTTTTTAATTCCTGGCCAAGCTCATTGTCAACTCAAAACTCTCAAAAGAACTAGTCAAAGCACACGGTAGCCTCGAAGGAGTAAGGATGTGAAGACGAAGGCTGCCTGCTTTTCAAAATTAACAGTTTTGAACTCTTTAAACCAAATTCACATTTTCTCCACTGCTTTTGTATTTCATGGTTT

 

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