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CRIMIC CR71632

Gene CG2955

CG2955

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:4149279..4151203	24D6-24D6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAGATGACACAGGAACAACCAA	CAGACAGACACTACCATTCACAGC

Images

Construct Design Sequences

sgRNA sequence	ATGTGTTCGTGTCACTCACTTGG
sgRNA2 sequence	GAAAATGAATACCGATTAATAGG

 

left_200bp	right_200bp
GGTCAGAGGTCACGAAAGAGTTAAGTAATATATATTTCTAGTAAGAATCTATACAAGAAGTACGATGTTTGTAGCACCGTTTAAACCTTAACATATCAAGAAAGAATAGCGACACATATTACAGATTTATTTTCGATCAGAGTAGGCTCAAAAGTACAAAAGTCAACATTTGTATGCTAATGCTTTCAGTTTAACCAAGT	AATAGGAGATAGCAAAGTTTGAAGTTTGCATAGAATACACTTAGATTTAAATATTTATATATGTACGTTTTCGGCCAAGTTGTTCACTAAAATTCCGTAAATCAGCTCGAACAAAATAAATTTTAAAGTATTACCACCCAAAATAAAATATGACCAACTAAAGATTTCCCCAGCTGTCGTTGTTTTAGCCGCAGTTGAGT

 

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