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CRIMIC CR71633

Gene Mcm7

CG4978

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:8891596..8894310	66E3-66E3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGCTCCGGACATGTAAG	GGATCATGGGCTGTTCTCGTAACT

Images

Construct Design Sequences

sgRNA sequence	AGCGTCCGAAAAAACACCTTTGG
sgRNA2 sequence	AATGTCTGGCAGGTCAACATGGG

 

left_200bp	right_200bp
ACAATTATCTATAACAACAATTTTCATTATACATTCTACATATGTAATTATGTAAAAATATAACGATAGTTTTATATTTTTAATTTATCTCTCATCTCAATCTATAGTACCATCTCTAATCAAACGCGTTTTTGGCGGGAAGCGTTTACATTCTCATTGTTTTCCCCGCATTTCCCAGCAAACAGCGTCCGAAAAAACAC	CATGGGACGCACGAAGATCACATTCATGTAGATCGCACGCTTCATCCCACATCTTATATTTAGTAAATAAGTTTATGTCTCTTTATCGCCAATCGTTAAAACTACTCTCATACATTGTCACTTTACCATTTATCATTGTCATAAATAAATAAGAATTTTGTATTTGACTTGAGGAGTGTGTCTCCTTTGTTCCAAAATGT

 

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