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CRIMIC CR71634

Gene mRpL18

CG12373

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:12756434..12757173	49C2-49C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTGCTTGCTTCTTCACCCAACC	TCTACAACACCCTGTTCAAGCGCA

Images

Construct Design Sequences

sgRNA sequence	TGGCAGTCTGGTAACACTAAAGG
sgRNA2 sequence	AACGAAACGATAACTAAGTGTGG

 

left_200bp	right_200bp
GGCGCATGTCTGAAGGGGGCGTTATTGTGGTGGGGAGCGGCGAAAGTTCCAAGGCCAATAGAATCTACAGAAACTAGAAATTTGAGGATAAACCAATCTGAAACAGCTGTTTTTTGCCATTTACATCCAACTGATGTTTGGACATTTTACAGCACCAATAAATCGTTTACCCACACTCAGTTCTGGCAGTCTGGTAACAC	TTAGTTATCGTTTCGTTTAGAATAAACGTTGATTATTATTGCTAATTACAACAAATCATGTTAATTTATTCGTATTTGCCCTTGATGTCCTTCCAGAGTTTCTGCAAAGCGGGGAAATCTTGAGATTTGGACAGGAAACTTTGCTTACATAACAGGATTATACTTACGCCTTTGTTGATGCCCTCGAAAATCGCAACCGA

 

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