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CRIMIC CR71637

Gene barr

CG10726

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:20058163..20061370	38B1-38B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTCAGCACTGGCAGGAC	CAGTGTGCACCAACACAAGTATGC

Images

Construct Design Sequences

sgRNA sequence	TGAAATGCAGCCCTAAATTGCGG
sgRNA2 sequence	AAAATAGAGGTAAGAGATTAAGG

 

left_200bp	right_200bp
TTCGATTTCGAAGCTTTAAGGTGTAAATTAAGTTTGTTTATTTCATTTTTCTCATATGATAAAATTATGTATCATGTTTATACTAGTTAATTATATTCCGCAATACTTTATAGCTAATTCTTTAGCGATTTTCCTGTCCATATTATCTAGCTAGCTTGGCACACCTTTAGTTTTAACCAACACTGAAATGCAGCCCTAAA	TCTCTTACCTCTATTTTTAACTCCCAATTCGTATCAGTATTCTGAAACAGATATCCTCACCCCAGTCGAATTTTATACACAAACGAAGCATTGTTCTGATTTAAACACTAGTTAGTAAACAATAAATTAGGAAATATCTAAGAAAATTTCGCTGTTTTAATGGGGTATAATAAACTGGTGAAATTTTCTTAATTGGTAAA

 

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