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CRIMIC CR71640

Gene atms

CG2503

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:4750035..4777490	82D3-82D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTGTGGGGTGATTTATCGC	AGCCAAGTGTAAATCGACTAGG

Images

Construct Design Sequences

sgRNA sequence	ACTAGAAGCGCTACCAAAACTGG
sgRNA2 sequence	TGTAATTAATCATTCATCTGAGG

 

left_200bp	right_200bp
TATCCATAATATTAGTTGTGGAAATGAAATTCAAATAGATGTTGTGTTATATACGATGAGGATGTTGCATTTGAGTCCCCGGAAATATAGTATTTTTTTTACCGAAGGTATTATCGTACCGGTCAAGTACGGTCACACTGCCAAGCGCAGATTTGAGGATTTCTAGATTTGGCCTCTTGATGGACTAGAAGCGCTACCAA	ATGAATGATTAATTACAAAAAACGGCGTTCATAATAAATAAGTTTATAATCAACCAAGTACATTTGAAAACTGAACTAACTCGATTTAATATCATTTTCGCCTCAACTCAGCGCTCGGGTTCGTTGCCCAGAATAGTTTTAAATAAAATCGGCAGTTTAACATAATTTATATTAGATGTTGTTGTTGTATTGCAAACAAG

 

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