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CRIMIC CR71645

Gene Pfas

CG9127

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:6041181..6046050	26B3-26B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTGCTGCAGGCGATTCCATCGAT	ATATGGCGTCATCAACAGCCA

Images

Construct Design Sequences

sgRNA sequence	CGAAGGATGACCATGTCGTGGGG
sgRNA2 sequence	TGTTTAACAACGCGTACAATTGG

 

left_200bp	right_200bp
CGGCTGTAACCTGATCCGCTGCTGTGGGTTGTGGGCTGTGTGGCTGTGGTATAGGTGCCGCCAGTTGATAACCCCCAGCGTGGGCACGGACCCACTGGTCGTCAACCGCTTCGCAACTCCCAAGATCATTCTCGATTCGACGAGCAAGGAGTGCACACTGTCTGTACTCAATCCGCATTTTTCGTTTCTTGCAGCCCCAC	AATTGGTGTGTCAAGTCGAATCAATAGATATTCTAGATGAATTGAAAGCAGTTAAATAGGTTCTTGGCATTTATGACCTGATATATCTAAATACTATATACACGAATTTGTTTACTATTTTTATAAAATAAACTTAAACACTCAAACAACTTTAAAACTCAAATAAGCGTACTTATGAGTAATGTCGTCGCAACGAATAG

 

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