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CRIMIC CR71646

Gene sktl

CG9985

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:20827282..20836028	57B3-57B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGCAACAGGTGCTCCAAAGTCTC	TAGCGTGTCTAGTTGGCAGTCA

Images

Construct Design Sequences

sgRNA sequence	CGGAAATCCCCAGAGGGAAATGG
sgRNA2 sequence	ACGAGCGTGGACGTGTAGCTGGG

 

left_200bp	right_200bp
TTGTTGCCTCTGGCTTAATCGATTACTCACCTACTCAAGCCCACTTACTCATCCACTCCACACTCgCAAACACACTCACATAAACACACCCACATACATACGCCCATTAATCGAGCTGCAATTGAATGCCTTTTGTACTGTTTGAGCGGCACTGCAATAAGTGGTTTTCTCCTCCGCAGAGAGCGGAAATCCCCAGAGGG	TACACGTCCACGCTCGTGCTCAACGATTTGCCGCGTTGATTTTCAGTGCGGGATTGGTCGGGATATGAAAGGAATCCTGAGCAGCAGCAGGAGCATGTATATATATAGCCCAAATGTGTATGTGTGTCTGCAAGGTGAAAGCGTCACTTCCACGCCCAAAAAGGGAAATTCTGAAAACGAGCTCGTTTATCTTGATTAAA

 

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