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CRIMIC CR71649

Gene Pp1-13C

CG9156

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:15359101..15361061	13B9-13B9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAATTCCATTCGGTTATGTTCTGG	CACGAACAGATGGAGGTGAATCTC

Images

Construct Design Sequences

sgRNA sequence	GGCCTCGGTCTTGGGGGCCGCGG
sgRNA2 sequence	AATATGCAAGAATTGGCTAGTGG

 

left_200bp	right_200bp
ATCATCACAAAAAGTGCACGTCATCAGGACATACATTTTGCTGCACCCAAGAAGCTAAGTTTGTGACCCAAATAGACCAGCTCCGCCGAGGATCCAGTCCCATTCTCCAGACAGCCGCGATCTCAGGGTGAGTCCCTGACAAGCAGCCCCAACGGCGTCCCAGACGATTGTCCTATCCAGCCCGGACAACGCAGCCGCGG	GCCAATTCTTGCATATTGTATGATATGTGTAAGTTCACTGAGATTTGAACGCAGAGCTTATGCTTAGCTAATGCAAAATAACGCCATATGCGACTTTTACAAAAATAAACTTACATTTAAAATTTCAAGTGTTTTCATCTAATTGTTGTTTTTTTTCGATTTCCTAATTAATCTTGTCAGCTAGTTTATCCAACTCTTAA

 

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