CR71652

CRIMIC CR71652

Gene PpY-55A

CG10930

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:17955396..17956570	55A2-55A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTCACTACACCCCATTCACGCC	AGTCAACAAGGAGTTGACTTCGGG

Images

Construct Design Sequences

sgRNA sequence	ACAAATAAAATCGAATACGATGG
sgRNA2 sequence	CCAAATGCCGACTAACGAAGAGG

left_200bp	right_200bp
GGCTAAAGTCGGATGTGGATTTTGTGGAAAGTGCGGAGCTGGAAGATTATCCTCACATATATAAAAAAAATAGTCTTAAGCGCTAAATATGTTTAATTTCACTAATACTATTACAAACAAAAAACACTTCCTTTCTAAAAAATGTCACAGTATATTATAAAAATTTTTCGTTGTATGTTTAGTACAAATAAAATCGAATA	AAGAGGAGTGATTTTGTTTTTCATACATGTATATATCACTTCGTGCAATTAAATTCGTACAATAACAATTCTTTTTATGTAGCTGATATATCAAATATATATACTATATATACGTTCACATTTAAATATTTAACCAGAAAATAAAAGACATTAGTTACTAGTATTCCATTGCATTTCTAAGTCTTTCAAATTAAGCAAAC