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CRIMIC CR71656

Gene Rpn10

CG7619

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	III:21537000..21438582	78D5-78D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TACTTTTCCACAGGACTAACATGGACTAAC	AGACATAATGAATATGTGGCATGGG

Images

Construct Design Sequences

sgRNA sequence	GTGTGCCGTTGCAAGCAGTACGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAATATCGCACAACGGTCATACAAATCCGATATTTTAATGCCGATAGTTCAGGCAGCACAGCACCTGGCAACCCTGGGGAATTACAGTTCAGTGGCAAAGTGACAAGCAAAAATACGAAAATGGTTCTGGAGAGTACTATGATATGGTAAATTATTGTTTTTTGAGCAATACGAAGAGCTTCCGCTAGTTTTCCCGTAC	TGCTTGCAACGGCACACAGTCTGGCACTCATATTTTGCTCTTTTCCAGCTTTGATAACAGCGACTTCCAGCGCAACGGAGACTACTTTCCAACTCGTCTCATCGTCCAGAGAGATGGCATCAATCTGGTCTGCCTGACCAAGCTGCGTTCCAATCCCGAGAACAATGTGGGCCTGATGACTCTGTCCAAGTGAGTGTGGA

 

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