( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 28
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 29
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 30
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 31
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 32
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 33
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 34
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 35
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 36
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 37
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 38
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 39
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 40
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 41
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 44
Call Stack
#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

CR71656

CRIMIC CR71656

Gene Rpn10

CG7619

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	:..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
		TACTTTTCCACAGGACTAACATGGACTAAC	AGACATAATGAATATGTGGCATGGG

Images

Construct Design Sequences

sgRNA sequence	GTGTGCCGTTGCAAGCAGTACGG
sgRNA2 sequence

left_200bp	right_200bp
AAAATATCGCACAACGGTCATACAAATCCGATATTTTAATGCCGATAGTTCAGGCAGCACAGCACCTGGCAACCCTGGGGAATTACAGTTCAGTGGCAAAGTGACAAGCAAAAATACGAAAATGGTTCTGGAGAGTACTATGATATGGTAAATTATTGTTTTTTGAGCAATACGAAGAGCTTCCGCTAGTTTTCCCGTAC	TGCTTGCAACGGCACACAGTCTGGCACTCATATTTTGCTCTTTTCCAGCTTTGATAACAGCGACTTCCAGCGCAACGGAGACTACTTTCCAACTCGTCTCATCGTCCAGAGAGATGGCATCAATCTGGTCTGCCTGACCAAGCTGCGTTCCAATCCCGAGAACAATGTGGGCCTGATGACTCTGTCCAAGTGAGTGTGGA