CR71658

CRIMIC CR71658

Gene PGRP-SC2

CG14745

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:8776950..8717095	44E2-44E2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTTGATAGGTCTTAGGCTGCAC	TTGATCAACTGACTTCCATAGAGATTCC

Images

Construct Design Sequences

sgRNA sequence	ATGGTTGCGGATCGATTGTGCGG
sgRNA2 sequence	TGCATTTCCTTAGTGCTCTTAGG

left_200bp	right_200bp
AATTAATTAATGCCACTTGGGCTGATTAGAAGCGAGATTGGGTCTCCGTCATCGACGTATCAGGATCTGGCCGGCAGATAGCCCTCCTATCAGATGGAAGCACACGACCCGGACCGTAGCCCGAACCTGACACATCGGCTCGCAGGCGATAAGCTGGGCCGTTCGGAGTATAAAAGGTCCGATCGGACGACAGGCCGCAC	AGCACTAAGGAAATGCAGTAAAAAAAATAAAAATCAGTTCCAATAAAATCTTAAGAATACAATCGCACACAATAATATAGTCTCGGGTATTTTTCTCCGGGGAATTATTTCTTGGGAACACCAACAAATCTTATTATTAGTCATCACTTAGTATATGCATACATATATGTAATGATCGGGAATTTTTATGTAGCTTCTTT