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CRIMIC CR71659

Gene IM18

CG33706

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:23600914..23601773	59F4-59F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAATCTAACCGACCTGACC	CGTGCAACAGGAAACATGGCTTCT

Images

Construct Design Sequences

sgRNA sequence	AATGCGATCAGCTTCATGGTTGG
sgRNA2 sequence	ATTGTTTCATTCTTTACGTCAGG

 

left_200bp	right_200bp
GGTGAATTCCAATCGCGAGAGGAAGGAGAGAAAATCTGATCGACGCCCCCCAGCTGATAAGAAATCACCCAAAGTCGGTGATTTCAGCGAATAGCAAAATCCCCCCGGGTGCTTCCCAATCCCAAGGGCATACGAGTGCAGCGTATAAAAACAAGCTATTGCCGGAGGTCGGCATTCAGATTCACTTGAGATTGCCAACC	GTAAAGAATGAAACAATAAAGATTTGAAACGCCTAAACTAAATTAATCGCCCAGTTTCCTTATCTTACTTATGAAATGGCCTTGATACACATCTGCTTACTATTTATAAAATGCGTAATAATTATTTCTAAATTTATTGCAAATTCTAAATTTCATATCGAAGTAGTTGCTTTTGACCATACGTCAAATATCCACATTGT

 

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