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CRIMIC CR71661

Gene Gnf1

CG1119

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:4906622..4910081	82E2-82E2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGCAATTCACCGTTGCG	CCATCACTGATCGCGTTACCAACA

Images

Construct Design Sequences

sgRNA sequence	ACAACTTACGCGTTGCATTACGG
sgRNA2 sequence	AAACAAAAACTACTTACAAATGG

 

left_200bp	right_200bp
CGACTTTATACCTTTTGTATCTTGACACACATTTAGTGAGCTATCGATAACGTTCAGTGCTTTTGATTCTTTAAAATACTTATACTAGTTTTTTTCAATCGTCACCGACGCGGTTACACTGGGGATTTCGTGTCTGGTAACACTGTTCCGTATTCTGAAGCTCGCAGCATTAATTTTATAATAATTAATTCCTGCCGTAA	GTAAGTAGTTTTTGTTTAAAATCTCATGAATGGCCGGCACTGGCCGACACTGTTTATACTGCTGAAAATATATATTTACCAATAAAATATGTGCTTGAATTGCAAAACATTTTATTATAACACTGAGTGGCGTAGGTGTAAACTAAAAAGGGAAAAATTGGTAATAAAATAATTGTCTGTATGAATAATACTACGAAAGA

 

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