CR71665

CRIMIC CR71665

Gene Septin5

CG2916

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_kG4	II:7960133..7961921	43F8-43F8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGTACTGTCGCAAGACTATCTTTCTGAT	TTAGGGCTGGATTATAGAGAACGCTG

Images

Construct Design Sequences

sgRNA sequence	CAGGCGCCACTCTTTATACCCGG
sgRNA2 sequence	ATTAGTATACTTCTTGGTATAGG

left_200bp	right_200bp
CGCGCAGAACTGTCTCGATAGACAACTGAGGCGGGCACCGCATTTAAAATCGTCAAGGCTGCAGGTAAAAGGAATCACAGGTAGAAAACTCTTAGATACAGTTTAATTCTCGGTACGCCACCAGATGCAGGAAAATCCAAATGGCTTATTAAGAAAAACGACTAGGGTTTACACAGGCTGCACATACGAGAATTCCGGGT	CCAAGAAGTATACTAATTTTGATATTTTATTTCTTTTTATTTTAACGATCTATGATTTCCAATTAAACAGTTTATAAATAAATTATATTGAAAGAATTTTAATTTAGGTTACGAGTTCCAGTAATTTTAGTTCTATAATTAAATACAATAAAAATATAAAAAACAATATTGAATGCGAATATGTCTAGCATATGAAAAAG