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CRIMIC CR71672

Gene PPYR1

CG15031

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:15243859..15246913	13B3-13B3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTACCAATGCACAAGAAGTACATAAC	GAAACAGGCGGAAACACAACATGG

Images

Construct Design Sequences

sgRNA sequence	TACGTTCATACTGTCGTCGAAGG
sgRNA2 sequence	TTAAATTTCAACACTGATCAAGG

 

left_200bp	right_200bp
AACTTCTGGCTTATATTTGCTGAGTCAATCTTAAACCGTTTTTGGGAAAACTTAGTTGCCCAGTCGATCAGCTGCCAGGCTCATTCCGTTTCTCATTCAGTTCAGCTCCAAACTCGCTCGCCTTCAGAGCTCCGATTATATAATAAATTATATATCTGATTTTTACTTAAATTCGAGCTTTAACAATCGTTGCACCTTCG	TCAAGGTGTTATTCCTTATCGCTTAAAATTCATTTAATTTGCTTGCGTTTGCGTGTTACATAGTATTAAATCGTTCAAAATTAAATAATCCATGTATTTTGAATAATTTCTACGTTCTTTAGGATATATATAAATCATAAATACATATAAAAACATAATCAAATAAAAATTTTATATTAGTTTATCAAGCTTTTAGTGAT

 

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