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CRIMIC CR71673

Gene Spn31A

CG4804

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:10060816..10063925	31A2-31A2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCGGATGCAGTGCGTCGAATTC	CGGAAATGCAAGGTAAGATTGTGG

Images

Construct Design Sequences

sgRNA sequence	GTCGACTAAACGCTTGCGCTCGG
sgRNA2 sequence	GGTCACATTCTCACTGCGAATGG

 

left_200bp	right_200bp
TGTGTGTGGCAAAACAAATTATAAAAATAGATTAAAAATTCAGCATGGGCAAAGTAGTTGCCGAAAATTCGAACTGAATTGTGGAAGCATTGGCCGCCATCCGGAGCTTTTGCCTCCGCGTTGCCCAAgTTGTCGTCACCGATGGATCGGATCTATGTATcCAATTGGTTCTTTCTCGAGCTATTTTATTATTTCCGAGC	GCAGTGAGAATGTGACCTATCTGATGGGTCACGTGGTGGAATTTTAAtAATTCTaAAAATTTAGATACACGGAAGGGTtGTGCAATATGTTAACTTgTATAAAACTAAGTAAAATAAAACTAATATTTACTAAACAAGTAAgtTCGATGTATGAATGCTTCGGAATATATGATtGATTTTTTGTATTTGTTGCAAAATGA

 

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