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CRIMIC CR71674

Gene Spn42Dc

CG9455

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:6880995..6883407	42D6-42D6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCACCTAGTAGCAGTTGTAAGC	CGAATTCAACGAAAGCGGATAGTC

Images

Construct Design Sequences

sgRNA sequence	GGGTTCGCTCATCTCGATCTGGG
sgRNA2 sequence	AACTGCGCACACAGCTCTCTCGG

 

left_200bp	right_200bp
AATTTTGAAAATGTTTTATTTTATTAAAGATTGAATCCaAATTCGAAATTGTTCTGtATTGATCAAGTTGGCGCTTAACAGCTGGCGATAATGCCAAATGAGATAATGAGATTTGGCCCGAAAATGTCTTATTTTTGTATACTTATGTTACATTTAAGTATTTAAAAATATAAATTAAAACATATATTTTTTTGCCCAGA	GAGCTGTGTGCGCAGTTCTCAGTAACGTAATGAAGTATCGCCAGTAGGTACATATATGTCCAGTGCATATATGAGCTCGTCTTGGCACTCGGCGCTCAGTTTACAGAGATACTTATCTCGGGGAAGAGTTCGTTGTAGACGGTCAAATGTATTACTTGTGTAAGTGGCGATCGCTTATAGCCAGTGAATTAAAATACAAA

 

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