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CRIMIC CR71676

Gene Mtr4

CG4152

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:15752263..15755900	35D4-35D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTACTTTGCCCATTTTGCGCTGC	CAGTGTGAACCGTCCAATAACTTATG

Images

Construct Design Sequences

sgRNA sequence	ACCATGTCTATCGCGTTATCGGG
sgRNA2 sequence	ATTTAACATATCGACTCAAAAGG

 

left_200bp	right_200bp
ATCTACATGTTATTAGTCTTCGAATTAAATTCGAATATACCAATCATATTTCCATAATAAAGTACAACATAATTTCTAACTATTATAATTTATTTTCTTTTATTTGAACTAATTCAAAAATAGCTTAATTTCCCCTCAAAATAGACAGACGGTCTTCACTGACGATGGTCGGCAGAATCGATAACCATGTCTATCGCGTT	AAAAGGTCGAAATATTTGAAaCCcAACAACCTTTGTAAATTTATTTAATGATAAAATGTTAATTTTATCCACGGTTAATAAAATAAATATGACCATGAGATCTAACATCTATTTACTTAGAAATCACGTTTGTTTTATTTCAAAGCCACAACAGTGAAgTCAAATTTGTCATAATTGATAATGTGGATATTCAAAGCATT

 

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