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CRIMIC CR71678

Gene maca

CG5213

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:15494602..15495620	88F6-88F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCCAACTCTTGACACCAC	CATAACCGAACGAATGCGTTAAGACC

Images

Construct Design Sequences

sgRNA sequence	CAGAGATCTTATGCTTTCTTCGG
sgRNA2 sequence	CTCTCGCGACGCTTGTAGGGTGG

 

left_200bp	right_200bp
GTTGGTAAATAACCAAGTAATTGTTGACCAAAGCTATCCTGTTTGTCATGGAAGTCATTGTCAAACACATTCTGAAAAAAAACTTTTAACATTTGGAAGGGCAGCTTTGCAGTATTTTTAGAATTTTCAGTCAATTTGGGATATTATAATCATTCTAAAATTTTCTACGCCAAGTGTTTCATTTGGATCCCATTCCGAAG	TACAAGCGTCGCGAGAGGACGAACGATCATCATGTGTCTAAGCGTTCTCGAGATTCGGACTAATCATTATGCGTATTTTTGATAGAACTTGCTGATTAATTTGATTAATATATATATAGAACCTGTAAGGAACACCTTTTTTTAAACCTCAGCTGAAGAACAAATTGTGATTTTGCACTTTTGTCGTATACTCAATTTCC

 

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