CR71682

CRIMIC CR71682

Gene Rat1

CG10354

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:6908083..6911423	27C4-27C4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTACATCTGACGATCTGGCATCCC	CTCGCATAGTTGTAATGAGTATTGTTTTCG

Images

Construct Design Sequences

sgRNA sequence	CATTGATCGGAGCATGCTTGTGG
sgRNA2 sequence	GAACTTGGCATGAGTTTGTTTGG

left_200bp	right_200bp
CGCCAAATTGTACACCCTTTGGATGAGTAAAAAATGGTTAGATAAATCGTTGAACTATTAATATTTACAACTTTTTAGGATAATTATTAGTCACTTTATTAAATTTAATGCCAATACTAATGGCATACATTTTGTATTTTTTATATGTTTTGGTATTTTTTCTCCCCGCCATTTGTACGGTCCCATTGATCGGAGCATGC	AAACTCATGCCAAGTTCTGAGTTTGTTCTTTAAATGAAATACCCATTGCATTTCTATCAAAGCAGCTTACAGCCAACATCTAGTCTTAGTGCCTCAAAGAAAATAAGTCAAATTACAATAATGATCATTTCTATTTCCATTGATTGCGTAAAAAAAGCAAACTAGCTGGATAATACAAAAAAATGAGACGGTTGTTATTT