CR71685

CRIMIC CR71685

Gene Su(var)2-10

CG8068

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	II:9116122..9122000	45A8-45A9

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGTGCCTTTCTACTTCACG	AAAGTCATAAGCGGAATCGGG

Images

Construct Design Sequences

sgRNA sequence	GTAGGAATGACATTCTGCATTGG
sgRNA2 sequence	ACACTGACTCAACCACGCTTCGG

left_200bp	right_200bp
CATTCGCAACAGCTCCAAGGTGGAGCACGCCATTCAGGTTCAACTGCGCTTTTGCCTGGTGGAGACTTCGTGCGACCAGGAGGACTGCTTCCCGCCGAACGTAAACGTCAAAGTGAACAACAAACTCTGTCAGCTGCCTGTAAGTATTATCCGATTATCCAATCAGGACCTGGTATAATATCCTGCTCTTTATTCCAATG	CTTCGGCACCAATAATTTAGACAAAGGAATGAAATCCTAACAAGATCAAGCATTTAGGTTTCGGTTCACCTTTTACGGCACACCTCTAGGACATCATTTAACGTAAAAGTATTTTTTTGGTGCATCAATTGTCTTAAATAATATTAGAAAATTTTAACTGCAAAAGTTCGAAAGAGTATTCACATTGTCCAAAATGAAAA