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CRIMIC CR71686

Gene Spt-I

CG4016

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:13168498..13170744	49F4-49F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGTAAGAGAATGTTGGAGAAGGG	CTGCTCCACTGGCAATCAAATCGT

Images

Construct Design Sequences

sgRNA sequence	ACGGAACCAGACTGTCAAAATGG
sgRNA2 sequence	TTAAACAATTCATACTGGACTGG

 

left_200bp	right_200bp
TCGCCCACTGGCAATTAGCCTATGTCAGCATACACACCCAATTGAAAAGATTTCCGTACCGTATGGTCGAATGCTACAATTCTACATATGTATGGGGCATACCCGCGATAAGAAAGCGTCGGCTAGATGACACATAACTTCAGGGAATTAAGGCGGTTTCAATTGATTTTTCACAGAAAGGGCACGGAACCAGACTGTCA	CAGTATGAATTGTTTAAGTTAATAGCTAGCCTTGCACTTGCTCTTTGCACTTTGTTGCATTGCATTTTAATGAAAAATTTTAAGTCTAGGAAAACTTTCAAAGATTACACGTTATAGATAACAGAGCTGGTTAAATAAAACTATTAAGTTTATTTGGTAGTTACGTGCCCTTTTATTGGTGTTTCTTATCGCCTGCGCAA

 

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