CR71687

CRIMIC CR71687

Gene Kdsr

CG10425

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:25260963..25262498	96C8-96C8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTTTGGTTGGAGCAGACACATG	GCTTTAGCGATTAGCACAATCACTAAG

Images

Construct Design Sequences

sgRNA sequence	GATTCTCTAATCTCTTACGCTGG
sgRNA2 sequence	GGGCACTTGGGAATACTTTAAGG

left_200bp	right_200bp
GCCATTGCACATTTAATTTATTATGAATAAAATTAAACAAAAAGTTTTGAAGAACAAAATATTTGCTTGGCCGCTTATAGCCACATTTGCATCTCTGATGTGCGTCGAAAGCTTTCGATAGAAATCCGGTTTGCGTCAACGGTCACACTTAGATGCACTTTCTAATCAGACGTTTAACTTTGGGTGCAGATAAGCCAGCG	AGTATTCCCAAGTGCCCATAATTAAATCACTTTTTTATAATAAATCAAAATGTGCGATTGTTTTGCATGGTTAGCCTATTCAAATATTACAATTCCCGTATTATCATTCATTTTTATATATATATTTGTATGTGCATTTGAACTTTTTTATTATTGCCGGGCTAAGGTTTTAACTATTTGTATCGTGTTTTCTAGACTAG