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CRIMIC CR71690

Gene Cpes

CG4585

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	X:23862962..23865398	60A5-60A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTAGCTTTCACTCTTTGATAGCTGAG	TTGCAGTGCTGCCCATCAG

Images

Construct Design Sequences

sgRNA sequence	CCTCAAAGTGTCACCATGATCGG
sgRNA2 sequence	GAATGACAAAATGCATTAGTTGG

 

left_200bp	right_200bp
GATTTCAAAATTTCAGAAGGGGATTTCAAAACCCTGCATGTTGCTTGCCAAACTTTTTATGCCGTGCGCGGCTTATCTTGACAACTACTTCGCTTCGATAAACCATATTGATCAGCTTCACTTCTTTCCGCCCCACAGCTGCGCCCTGAAATCAGCCCGATAAGAACTTAGTGTTAAGCGCTACCTCAAAGTGTCACCAT	AATGCATTTTGTCATTCAAGCGCGGTCTGCAAGAGCAATCGAAACTCCTTCAGCTGTAAATAATAAATGTTATTGATTAATAAAGTCTAGCTGTTTTTATCGTACGCATTTCCATGACGAGCTAATTTCGTTTGAATTTATTTATAAAGTTTTGTGTTTCGAAATTTCAAATGAACACGATGCGCAATGCTGCCAAGTTT

 

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