• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR71692

Gene nSMase

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:3219156..2120925	63B14-63B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTTGCAAGACAAGCTTGTTCGCA	GGTAGCGACCAAGTACTGCATACT

Images

Construct Design Sequences

sgRNA sequence	TCACGTTCGCATGCTTACAATGG
sgRNA2 sequence	TGCTTATAGTACGCTACACGTGG

 

left_200bp	right_200bp
AATTAGAAATTAATAATGCAAAGAAATGCATCCCTGTTCATGTGCGACTTTGACTTGTCGCATCAACAAAGCTCGTCAGGGGTTCAGGCTTTATTAAATTTCGGCTTTCCCCAGACGGTCACACTGTGGCTTTATATCTTTTAAAAATAACAAAATCAAAAAGTTATCTTATCGCTGCTCCCGCGCCCGTTATTCCATTG	GTAGCGTACTATAAGCAGCGATATATGTTTGACTAGAGTTATAGCGCTCTTACTTGATCTTTTTATTCGTGTAACCGTCAATGCAATTCCATGGTCTCTTTCTGTGTAACGTCATCCATATCCCAGGAATAATAGTTCAATAAAAGTGAGAATCATTTAGGCAAACAAAGATTGATTGATCTAAAGATACCTTTTGTATC

 

---

Search Again